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Zwarte peper en overgewicht*
Uit een Koreaanse laboratoriumstudie blijkt de bioactieve stof piperine in zwarte peper wellicht goed werkzaam te zijn om overgewicht tegen te gaan. Piperine blijkt de expressie van genen, die verantwoordelijk zijn voor de aanmaak van vetcellen zodanig te beïnvloeden dat minder nieuwe vetcellen worden aangemaakt. 
Piperine, a Component of Black Pepper, Inhibits Adipogenesis by Antagonizing PPARγ Activity in 3T3-L1 Cells
Ui-Hyun Park†, Hong-Suk Jeong§, Eun-Young Jo§, Taesun Park#, Seung Kew Yoon , Eun-Joo Kim , Ji-Cheon Jeong*§, and Soo-Jong Um*† 
† Department of Bioscience and Biotechnology, BK21 Graduate Program, Sejong University, Seoul 143-747, Korea
§ Department of Biochemistry, Molecular Biology and Internal Medicine, College of Oriental Medicine, Dongguk University, Kyungpook 780-714, Korea
# Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, Seoul 120-749, Korea
Department of Internal Medicine, The Catholic University of Korea, Seoul 137-701, Korea
Department of Molecular Biology, BK21 Graduate Program, Dankook University, 126 Jukjeon-dong, Suji-gu, Yongin-si, Gyeonggi-do 448-701, Korea
J. Agric. Food Chem., 2012, 60 (15), pp 3853–3860
DOI: 10.1021/jf204514a
Copyright © 2012 American Chemical Society
*(S.-J.U.) Phone: +82-2-3408-3641. Fax: +82-2-3408-4334. E-mail: umsj@sejong.ac.kr. (J.-C.J.) E-mail: jjcjh@paran.com.
This study investigated the antiadipogenic activity of black pepper extract and its constituent piperine in 3T3-L1 preadipocytes as well as the underlying molecular mechanisms. Both black pepper extract and piperine, without affecting cytotoxicity, strongly inhibited the adipocyte differentiation of 3T3-L1 cells. The mRNA expression of the master adipogenic transcription factors, PPARγ, SREBP-1c, and C/EBPβ, was markedly decreased. Intriguingly, mRNA levels of PPARγ target genes were also down-regulated. Moreover, a luciferase reporter assay indicated that pipierine significantly represses the rosiglitazone-induced PPARγ transcriptional activity. Finally, GST-pull down assays demonstrated that piperine disrupts the rosiglitazone-dependent interaction between PPARγ and coactivator CBP. Genome-wide analysis using microarray further supports the role of piperine in regulating genes associated with lipid metabolism. Overall, these results suggest that piperine, a major component of black pepper, attenuates fat cell differentiation by down-regulating PPARγ activity as well as suppressing PPARγ expression, thus leading to potential treatment for obesity-related diseases. (Mei 2012)