Paddestoelen tegen kanker*
Steeds meer studies geven aan dat het regelmatig eten van paddestoelen zoals de gewone champignon goed is tegen het krijgen van borstkanker. In deze laboratoriumstudie werd dit nog eens onderzocht. Verschillende paddestoelen zoals de witte en bruine
champignon, de oesterzwam en de
maitake. Alle paddestoelen konden de celdeling in borstkankergezwellen duidelijk doen afnemen. Maitake zorgden ook nog voor celdood.
Commonly consumed and specialty dietary mushrooms reduce cellular proliferation in MCF-7 human breast cancer cells
Keith R Martin and Sara K Brophy
Nutrition Program, Healthy Lifestyles Research Center, Arizona State University, 6950 East Williams Field Road, Mesa, AZ 85212, USA
Corresponding author: Keith R Martin. Email: keith.r.martin@asu.edu
Worldwide, over one million women will be newly diagnosed with breast cancer in the next year. Moreover, breast cancer is the second leading cause of cancer death in the USA. An accumulating body of evidence suggests that consumption of dietary mushrooms can protect against breast cancer. In this study, we tested and compared the ability of five commonly consumed or specialty mushrooms to modulate cell number balance in the cancer process using MCF-7 human breast cancer cells. Hot water extracts (80°C for 2 h) of maitake (MT, Grifola frondosa), crimini (CRIM, Agaricus bisporus), portabella (PORT, Agaricus bisporus), oyster (OYS, Pleurotus ostreatus) and white button (WB, Agaricus bisporus) mushrooms or water alone (5% v/v) were incubated for 24 h with MCF-7 cells. Cellular proliferation determined by bromodeoxyuridine incorporation was significantly (P < 0.05) reduced up to 33% by all mushrooms, with MT and OYS being the most effective. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) reduction, an often used mitochondrion-dependent marker of proliferation, was unchanged although decreased (P > 0.05) by 15% with OYS extract. Lactate dehydrogenase release, as a marker of necrosis, was significantly increased after incubation with MT but not with other test mushrooms. Furthermore, MT extract significantly increased apoptosis, or programmed cell death, as determined by terminal deoxynucleotidyl end labeling method, whereas other test mushrooms displayed trends of 15%. The total numbers of cells per flask, determined by hemacytometry, were not different from control cultures. Overall, all test mushrooms significantly suppressed cellular proliferation, with MT further significantly inducing apoptosis and cytotoxicity in human breast cancer cells. This suggests that both common and specialty mushrooms may be chemoprotective against breast cancer.
This version was published on 1 November 2010
Exp. Biol. Med. 2010;235:1306-1314
doi:10.1258/ebm.2010.010113
© 2010 Society for Experimental Biology and Medicine (November 2010)
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