Granaatappel tegen overgewicht.*
Uit een studie met muizen blijkt dat extracten van bladeren van granaatappel de vetopslag en de eetlust sterk deden verminderen bij muizen met overgewicht. De eetlust bij muizen met normaal gewicht veranderde niet. Na vijf weken een speciaal dieet aangevuld met de extracten hadden de muizen duidelijk minder gewicht en duidelijke lagere bloedwaarden aan suiker, cholesterol en triglyceriden.
Evidence
of anti-obesity effects of the pomegranate leaf extract in high-fat diet induced
obese mice
F Lei1,
X N Zhang1, W Wang1, D M Xing1,
W D Xie1, H Su1 and L J Du1
1Laboratory
of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology,
Tsinghua University, Beijing, PR China
Correspondence:
Dr LJ Du, Laboratory of Pharmaceutical Sciences, Department of Biological
Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China.
E-mail: lijundu@mail.tsinghua.edu.cn
Abstract
Objective:
To
investigate the anti-obesity effects of the pomegranate leaf extract (PLE) in a
mouse model of high-fat diet induced obesity and hyperlipidemia.
Design:
For
the anti-obesity experiment, male and female ICR mice were fed with a high-fat
diet to induce obesity. When the weight of the high-fat diet group was 20%
higher than the normal diet group, the animals were treated with 400 or 800 mg/kg/day
of PLE for 5 weeks. Body weight and daily food intake were measured regularly
during the experimental period. The various adipose pads were weighed and serum
total cholesterol (TC), triglyceride (TG), glucose and high-density lipoprotein
cholesterol (HDL-C) were measured after 5 weeks, treatment with PLE. In the fat
absorption experiment, both the normal and obese mice were given 0.5 ml
lipid emulsion and PLE at a dose of 800 mg/kg at the same time. Serial
serum TG levels were measured at times 1, 2, 3, 4 and 6 h after the
treatment. TGs in fecal excretions were measured after the mice were orally
given a lipid emulsion. Effects of PLE and its isolated compounds (ellagic acid
and tannic acid) on pancreatic lipase activity were examined in vitro.
Results:
The
PLE-treated groups showed a significant decrease in body weight, energy intake
and various adipose pad weight percents and serum, TC, TG, glucose levels and
TC/HDL-C ratio after 5 weeks treatment. Furthermore, PLE significantly
attenuated the raising of the serum TG level and inhibited the intestinal fat
absorption in mice given a fat emulsion orally. PLE showed a significant
difference in decreasing the appetite of obese mice fed a high-fat diet, but
showed no effect in mice fed a normal diet.
Conclusion:
PLE can inhibit the development of obesity and hyperlipidemia in high-fat diet induced obese mice. The effects appear to be partly mediated by inhibiting the pancreatic lipase activity and suppressing energy intake. PLE may be a novel appetite suppressant that only affects obesity owing to a high-fat diet. (Maart 2007) (Opm. Meer over granaatappels)