Omega-3 vetzuren versterken anti-oxidante werking*
Uit een Duitse studie over de gevolgen van de inname van de omega-3 vetzuren DHA en
EPA op de expressie van genen in het menselijk lichaam, blijkt dat deze inname leidt tot een sterk vergrote expressie van anti-oxidante genen waardoor de kans op ontstekingsziektes zoals hartziektes duidelijk verminderd. In de studie kregen 20 personen, 10 “normale”en 10 met sterk afwijkende hoeveelheden lipiden in hun bloed, 12 weken lang dagelijks een aanvulling met ruim 1 gram DHA en ruim 1,5 gram EPA. Nadien werd met speciale apparatuur metingen verricht van de expressie van de verschillende anti-oxidante genen zoals glutathion en de cytochroom P450 enzymen, waarvan er een aantal belangrijk zijn bij de afbraak van verschillende lichaamsvreemde stoffen.
Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
Simone Schmidt1, Frank Stahl2, Kai-Oliver Mutz2, Thomas Scheper2, Andreas Hahn1† and Jan P Schuchardt1*†
· * Corresponding author: Jan P Schuchardt Schuchardt@nutrition.uni-hannover.de
· † Equal contributors
Author Affiliations
1 Faculty of Natural Sciences at the Leibniz University of Hannover, Institute of Food Science and Human Nutrition, Am Kleinen Felde 30, 30167, Hannover, Germany
2 Faculty of Natural Sciences at the Leibniz University of Hannover, Institute of Technical Chemistry, Callinstr 5, 30167, Hannover, Germany
Nutrition & Metabolism 2012, 9:45 doi:10.1186/1743-7075-9-45
Abstract
Background
The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes.
Methods
Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR).
Results
Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis.
Conclusions
Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention.
Trial registration
ClinicalTrials.gov (ID: NCT01089231)
De volledige studie.
(Augustus 2012)
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