Resveratrol tegen schade aan bloedvaten*
De binnenkant van slagaders is bedekt met een laagje aaneengesloten endotheelcellen. Deze laag is heel belangrijk voor onder meer soepele vaten, een goede bloeddruk en als beschermingslaag. Regelmatig hoge bloedsuikerwaarden,
zoals bijv. bij diabetes kunnen echter dit endotheel beschadigen waardoor ontstekingen ontstaan in de vaten, het begin van
aderverkalking. Uit deze studie blijkt nu in detail hoe
hoge bloedsuikerwaarden het endotheel
beschadigen waardoor het endotheel poreus wordt en waardoor cholesterol, calcium en andere stoffen die
vervoerd worden zich in de wand kunnen vestigen. Uit deze studie blijkt ook in detail hoe de bioactieve stof
resveratrol in de voeding dit poreus worden van het endotheel volledig tegen kan gaan, ook bij hoge bloedsuikerwaarden.
Resveratrol ameliorates high-glucose-induced hyperpermeability mediated by caveolae via VEGF/KDR pathway.
Tian C, Zhang R, Ye X, Zhang C, Jin X, Yamori Y, Hao L, Sun X, Ying C.
Source
Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Abstract
Endothelial hyperpermeability induced by hyperglycemia is the initial step in the development of atherosclerosis, one of the most serious cardiovascular complications in diabetes. In the present study, we investigated the effects of resveratrol (RSV), a bioactive ingredient extracted from Chinese herb rhizoma polygonum cuspidatum, on permeability in vitro and the molecular mechanisms involved. Permeability was assessed by the efflux of fluorescein isothiocyanate (FITC)-dextran permeated through the monolayer endothelial cells (ECs). The mRNA levels, protein expressions, and secretions were measured by quantitative real-time PCR, western blot, and ELISA, respectively. Increased permeability and caveolin-1 (cav-1) expression were observed in monolayer ECs exposed to high glucose. Resveratrol treatment alleviated the hyperpermeability and the overexpression of cav-1 induced by high glucose in a dose-dependent manner. β-Cyclodextrin, a structural inhibitor of caveolae, reduced the hyperpermeability caused by high glucose. Resveratrol also down-regulated the increased expressions of vascular endothelial growth factor (VEGF) and kinase insert domain receptor (KDR, or VEGF receptor-2) induced by high glucose. Inhibition of VEGF/KDR pathway by using SU5416, a selective inhibitor of KDR, alleviated the hyperpermeability and the cav-1 overexpression induced by high glucose. The above results demonstrate that RSV ameliorates caveolae-mediated hyperpermeability induced by high glucose via VEGF/KDR pathway.
PMID:
22983702
[PubMed] (Februari 2013)
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